Coding

Part:BBa_K1799008:Design

Designed by: Michael Flanagan   Group: iGEM15_Genspace   (2015-08-07)


LsrC (modularization optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 706
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This version of the open reading frame has been optimized for modularization via silent mutations of the LsrD RBS, as well as any segments of code sharing more than 83% homology with the Shine-Dalgarno sequence. It is otherwise identical to the wild type LsrC gene (https://parts.igem.org/Part:BBa_K1799004).


Source

This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13807

References